comparison of sequences from 5'-flanking region - (Mar/13/2006 )
I want to compare 5'-flanking sequences of a particular gene from rat, mouse and human
can u let me know any method to do this
i want to do the comparison to find percentage similarity between both and what sequences are conserved
can u help me get started
-Watson-
are they in the Blast database? I would begin there, for starters
-aimikins-
You can use UCSC genome broswer which can show comparative genomics.
-pcrman-
Use the emboss programs, look at needle to do pairwise global alignments to get sequence identities and similarity.
To compare all three use emma (also emboss package).
HTH.
-DPK-