Help. Real- time against nested - I need to run a real time experiment (Mar/09/2006 )

Hello everybody!
Here the problem. I'm inserting a mutation (4 different nucleotides) in a cell line. The mutant cells are expected at a frequency 2 to 4 every 10 to the fifth cells. Actually the protocol says that I should diluite the cells in 96 wells plates at 5000 cells per well and check every well with a nested PCR (external one and one PCR allele specific). I'm trying to optimize everything in one Real time PCR step with molecular beacons. I made my plasmids with wt and mutant sequence and I can still detect my plasmid with the mutant sequence at 10 to the minus tenth (1 copy) which has a Ct away for Wt plamid at 10 to the minus third of about 1.8 Ct. Do you think I can detect even 1 copy of this gene or should I stay sticky to the nested PCR protocol? Who know if the real time is more sensitive?

Thanks
Micky