Trypsinization of RAW 264.7 cells - RAW 264.7 trypsinization without scraping (Mar/01/2006 )

Can somebody tell me if there is a way to passage/harvest RAW 264.7 cells without scraping.

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try 1:5 trypsin for first assays.
If don't succed, try to trypsin (no dilute) and to hit gently the plate. That helps great to detach cells.

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Im not sure if trypsinisation is ideal for RAW 264.7 cells as I have always split them by scraping. Is there a reason for not wanting to scrape?

Do you use non-heat inactivated FBS or heat inactivated FBS for your growth medium? Do you know if this factor affects raw cell viability much?

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I am not a fan of scraping either...
I am planning to try Versene (when I finally get it) since it is gentler than trypsin.

Yeah- has anyone tried culturing RAW cells in non-heat inactivated FBS?
I've heard that heat inactivated FBS can be actually disadvantageous to some cell lines

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