Plant leaf agroinfiltration method - (Feb/26/2006 )

I'm using the agroinfitration method to transiently express tagged proteins in N.benthamiana leaves. The Agrobacterium causes a microscopic damage to the cells by creating "bubbles" between the cells of injected leaf (like separating the walls of adjasent cells).
Did anybody encounter this problem, and any suggestions to avoide this?
thanks!
Raul

hi

i thought i replied to this , sorry if the reply didnt reach

when u infiltrate try not to get air bubbles in the syringe especially between the syringe and leaf tissue

i encountered such problems too while infiltrating .

take the culture full volume and at the tip of the syringe ( needle less of course) keep it filled and no air bubble


hope this clarifies and helps a bit

regards
laxmi