Combine fluorescense microscopy with HE-staining? - (Feb/24/2006 )

Hi,

I do fluorescense microscopy with monoklonal AB, Cy3 secondary AB, on tissue sections. Now I want to stain the hole slide to see the structure. I want to do that Haematoxilin Eosin staining. Is it possilble to combine the 2 methods, fluorescense and HE-Staining, or is there another staining method, that works better with fluorescense?

Thanks

Tom

-gigapackiii-

hi

since you can obtain a picture of your HE staining in the bright field channel, you would be able to merge others channels like fluorescence on it...

Seb

-tryptofan-

Hematoxylin is autofluorescent and eosin may be (I can't remember well about that one) so I think it's a bad idea to do both on the same section. I suggest you do as most people do which is use a nuclear fluorescent counterstain such as DAPI or Hoechst 33342 for overall structure or worse comes to worse take a phase contrast picture and do overlay.

QUOTE (gigapackiii @ Feb 24 2006, 05:46 AM)

I do fluorescense microscopy with monoklonal AB, Cy3 secondary AB, on tissue sections. Now I want to stain the hole slide to see the structure. I want to do that Haematoxilin Eosin staining. Is it possilble to combine the 2 methods, fluorescense and HE-Staining, or is there another staining method, that works better with fluorescense?

-MaximinaNYC-