primer design tools - primer designing (May/08/2002 )
If its a known gene you may a chance in finding the published primers inthese sites
http://web.ncifcrf.gov/rtp/gel/primerdb/
http://pga.mgh.harvard.edu/primerbank/
autosearch using primer5,then choose the ones of high volume,then to analyze in OLIGO, then you will get what you want.
Use BioInfoMan at http://www.bioinfoman.com/ to design sequencing and PCR primers automatically.
Kawaka
I found one at http://bioinfoman.com/bm/. It is the best primer design tool I have tried and it is free.
I just came across an allele-specific primer design tool called WASP which is integrated to Human Genome database.
http://bioinfo.biotec.or.th/WASP
I rather use Primer Express o Primer3 when designing oligonucleotides, but always run Oligo Analizer (http://eu.idtdna.com/analyzer/Applications/OligoAnalyzer/) when I wanna be sure about their structure 
what are all the tools used to design a primer<p>i have down loaded related databases from nlm.nih site
and i want to design primer.<p>thank u in advance
Write your own script or ask somebody else to is simplest way.
SmartNote is a free online lab notebook which contains many sequence analysis tools, including Primer3 and PrimerX, and also allows you to easily run them in batch. Check it out:
http://smartnote.miraibio.com
http://smartnote.miraibio.com
Many here:
http://search.vadlo.com/b/q?keys=primer+de...mp;sn=158621799
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