ChIP DNA purification - URGENT please (Feb/21/2006 )

hi every one

i read some where about using the qiagen gel dna extraction kit for dna puriciation after CHIP instead of using phenol cholofrom has any one used this? any other recommendations?

thank you

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Someone has used Qiagen kit for ChIP DNA purification, check it here
http://www.protocol-online.org/forums/inde...?showtopic=6929

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I've used Qiagen's PCR product purification kit as an alternative to phenol/chloroform, and found it worked just as well.

statler

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Yes, one of my colleagues also uses Qiagen spin column for ChIP DNA purification. I guess why companies selling ChIP kit (such as upstates) do not say you can use qiagen kit is because they want to sell their own columns at much higher price.

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Can I do ChIP with just 1 million or less cells?

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I believe so. According to Upstate protocol, you can use around 5x10^6 cells per IP. I did try this number of cells; and it worked.

Good luck-V

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5x10^6 is 5 million rite...i need to know 1x10^6 or less

thanks

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Using our Fast ChIP protocol (Nelson et. al. , Nucleic Acids Res. 34: e2, 2006) I've used that small a number of cells and still gotten decent results with QPCR. Just reduce the volume you elute in if you're trying to pull down proteins which don't bind much DNA.

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thx buddy

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