zymogram - (Apr/04/2001 )

Has anyone got any experience with zymograms?

Hi, I'm Laura , I know this techinque because I wrought whit enzymes like phosphatase.In brief my protocol is follower.After run whit SDS-Page , I kept the gel in buffer renaturant, for about 2 hours and than in another buffer for the develop, for 2 hours again.At the last step at this buffer I add an specific substrate and a few minutes I can seen the specific band relative at my enzyme.If you want a specific protocol, write me.Good lucky,Laura

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Hi, I have worked with zynogram, I jsut gointo this site . Do you still need the protocol?Srini

Hi, I guess my first msg was garbled. I do work with zymogram but then do you still do a protocol?Srini

Yes!