Southern Blot/ no signal - please look at the film (Feb/13/2006 )
I got the picture which is below after I did SB. The genomic DNA was the same at the both membranes. I have 2 membranes because first gel was melted little bit when I run it so I was afraid of the bad transformation.
You could clearly see markers (they're different) but there's no bands which I expected 
What could be possible problem here? All opinions would be more than welcome.
-suntoucher-
I would add a positive control for your probe, if you can, to ensure specificity
-aimikins-
QUOTE (aimikins @ Feb 13 2006, 03:39 PM)
I would add a positive control for your probe, if you can, to ensure specificity
that was SB for the positive control
-suntoucher-
QUOTE (suntoucher @ Feb 13 2006, 04:08 PM)
I got the picture which is below after I did SB. The genomic DNA was the same at the both membranes. I have 2 membranes because first gel was melted little bit when I run it so I was afraid of the bad transformation.
You could clearly see markers (they're different) but there's no bands which I expected
What could be possible problem here? All opinions would be more than welcome.
[attachment=587:attachment]
You could clearly see markers (they're different) but there's no bands which I expected
What could be possible problem here? All opinions would be more than welcome.
[attachment=587:attachment]
If you are using radiaoctive isotope, then more likely it is the unincorporated nucleotides that are binding to your marker bands. But it doesn't look like you are having any hybridization to your gene of interest.
Good lock.
Feruskas
-feruskas-