Help: Why do plaques stop growing? - (Feb/12/2006 )
I have a question in my mind when doing experiment.
When using virus to infect bateria in a plate, plaques form.
Why do plaques stop growing after reaching a certain size?
Thanks!
When using virus to infect bateria in a plate, plaques form.
Why do plaques stop growing after reaching a certain size?
Thanks!
better answer like this:
why you dont growth after 25?
think about it.
have fun
firstly akshik comparing the growh of one individual and a colony a vrus undergoing many generations might not be an exactly profound way to answer this question, aside people continue to grow after 25, cells continue to divide and die, so basic cellular growth is present.
As for plaque growth, is it not a case of incubation times, I mean if you leave these colonies for a longer period of time they should theoretically multpiply through out every available cell.
Remember that the apparent rate of growth of the plaque will decrease as the diameter of the plaque increases. You may still be getting the same rate of infectivity and cell lysis. If you left it long enough, you could get to (near) complete lysis, but you'd probably have dried out the plate by then, or else get contamination.
Why nottry it and see what happens over a week or so. Nothing quite like actually doing the expt!!!
true as the slight increases in the circumference during late infectionn will be difficult to visualise compared to intial changes, due to the area infected covering a larger perimeter
but what the heck stick those cells in the incubator and watch them dye mwuh haha mwuh haha
p.s. dont start other thread about cells and their right to live, as happened eon ago :-)
Good to see Oxford is still a safe haven for evil scientists
Keep up the good work! i always though, phages would stop multiplying after their host cells
have reached stationary phase...
even in stationary phase, the cell line is still producing a variety of proteins etc, which a virus can hi-jack to produce progeny, although a limitation of nutrients might slow down viral growth, I believe that the viral replication will make use of all possible mechanisms to produce progeny, in this case cells would dye but produe lower titre of virus