T-lymphocytes - (Sep/08/2001 )

Someone who knows .....please help me this.....Why is 2-mercaptoethanol sometimes added to the complete RPMI medium for culturing T-cells ?

Thanks a lot...

B-M, help mouse T cell stable of their membrane, prevent oxidize ...,you can find it on medline. nobody complete understand it.

B2mcan also have mitogenic affects

2-Me is sometimes added to complete RPMI (for murine cells only) because it is thought to act as a reducting agent thus facilitation antigen processing. However, the effects of 2-Me are generally unknown