how does a promoter work - promoter (Feb/01/2006 )
Hi,
i have been looking for a detailed information , but coudent ever get it , can any one please tell me how does a promoter works in the plasmid DNA , and what makes it specific or long acting or how it effects the gene silencing.
thanks a lot
what promoteur is it?
all promotor doesn't really function in the same way?
if it's a Lactose promotor it's generaly associated with the LacIq gene which is a lactose promotor repressor, so the RNA polymerase, because of the presence of the repressor on the level of the operator, cannot be fixed above the promotor without lactose or IPTG (isopropyl-b-D-thiogalactoside), so if in the culture there isn't lactose or IPTG the gene which is controlled by the promotor won't be translate. If you want produce the protein you have to introduce in your culture lactose or IPTG, which interact with the repressor and released the operator so the RNA polymerase will be able to fix above the promotor and the translation of the gene will start.
But there is different sorte of pormotors and they don't have the same way of regulation.
The regulation use in plasmide construction is the same as if it's where in the chromosome, when we made a plasmide all we do is a copy of what it's happen in the chromosome we just use the promotor and so the regulation interesting for us.
I think that you must surely will find information on the web.
all promotor doesn't really function in the same way?
if it's a Lactose promotor it's generaly associated with the LacIq gene which is a lactose promotor repressor, so the RNA polymerase, because of the presence of the repressor on the level of the operator, cannot be fixed above the promotor without lactose or IPTG (isopropyl-b-D-thiogalactoside), so if in the culture there isn't lactose or IPTG the gene which is controlled by the promotor won't be translate. If you want produce the protein you have to introduce in your culture lactose or IPTG, which interact with the repressor and released the operator so the RNA polymerase will be able to fix above the promotor and the translation of the gene will start.
But there is different sorte of pormotors and they don't have the same way of regulation.
The regulation use in plasmide construction is the same as if it's where in the chromosome, when we made a plasmide all we do is a copy of what it's happen in the chromosome we just use the promotor and so the regulation interesting for us.
I think that you must surely will find information on the web.
thanx a lot, i read information about IPTG but this is the first time i realy understand it....
