mesuring bans intensities - (Jan/24/2006 )

dear all, i have to deternine a certains protein half-life, so I have to quantify the relative bands intensities on a film (after a western blot) and "normalize" them using b-actin to demonstrate the gel was equally loaded,
does anybody know wich computer program should I use? And, just in case, if there are any for free on the web?

all the best for you all

-gonni-

ImageJ from NCBI does the trick quite nicely, just Google it and you can download the program for free. Beware, I find it easier to invert the image first so you get higher values if your band is more intense, it makes it a little more logical...

Greetz,
Karl

-ktvdepoe-

Hi,

do you want how ImageJ works?
click on the link to this topic:
http://www.protocol-online.org/forums/inde...?showtopic=8201

Sébastien_

-tryptofan-

You could also use the histogram function in GIMP (GIMP is also free, and a nice alternative to Photoshop).

-jaknight-