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Intron Splicing doubt - (Jan/10/2006 )

Does anyone know if the intron slicing is dependent on 5' UTR sequences? I'm trying to clone a genomic sequence (including introns) in a vector with an inducible promoter. My insert starts at the initial ATG and does not contain any 5' UTR region. Will this interfere with intron splicing or is the plicing independent of the UTR region and pormoter region?

-raco-

a colleagu who succesful expressed a protein did not integrated the 5' UTR.
plasmid contained the B globin intron and human growth hormone polyA.

-fred_33-

QUOTE (fred_33 @ Jan 10 2006, 04:08 AM)
a colleagu who succesful expressed a protein did not integrated the 5' UTR.
plasmid contained the B globin intron and human growth hormone polyA.



Thanks Fred! I'll keep myself optimist! I Hope it will work for me as well!

Best,
Raquel

-raco-