Difference between Genomic Vs Chromosomal DNA - (Dec/17/2005 )

How to differentiate genomic DNA from chromosomal dna in a gel.

regards
ravella

-Ravella-

They sound the same to me.

-pcrman-

isnt the same?

QUOTE (pcrman @ Dec 18 2005, 10:35 PM)

They sound the same to me.

-tertu-

In the gel they are same. Only in dictionary they are different!

-Molonco-

Or may be by telling genomic you refer to chromosomal DNA + Mitochondrial DNA? Plz clarify the question!

-Molonco-

Yea, genomic DNA is chromosomal DNA + mitochondrial DNA. If you refer to bacterial DNA which has no mitochodrial DNA, then the genomic DNA is considered same as the chromosomal DNA and there is no difference when being run on the gel.

-Angel1024-

QUOTE (Angel1024 @ Dec 21 2005, 12:06 PM)

If you refer to bacterial DNA which has no mitochodrial DNA, then the genomic DNA is considered same as the chromosomal DNA...

Not true. Genomic DNA refers to the entire complement of DNA contained within a bacterial cell -- thus genomic DNA includes plasmids. If the strain carries no plasmid, then genomic DNA equals chromosomal DNA, but it is more clear to then refer to it just as chromosomal DNA.

If a bacterial strain does carry a plasmid, it is frequently possible to differentiate chromosome from plasmid on a gel (depending on the size of the plasmid) -- if some form of the plasmid migrates differently enough to be seen outside of the chromosomal DNA band.

-HomeBrew-

Hi..

It's Quite correct...

But when U come to c Extra chromosomal DNA ( Mitochondrial, Chloroplast.. in Eukaryote and Plasmid un Prokaryotic) they won't coming under chromosomal DNA, but coming under genomic DNA.

If the extra chromosomal DNA is a meaningful word the above suggestion is correct.
Is it correct....?

Bye
Siva

-shiva-