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real time PCR - getting high peak with c-ve (Dec/07/2005 )

hi! I'm getting very high peak of my c-ve in my real time PCR and when I run the products from capillaries in gel electrophoresis I don't get bands in c-ve please I need help!

-fgalil-

any help

-fgalil-

What is c-ve?

-Excentrifuge-

amplification peaks or melting curve peaks?

I bet it's primer dimer, but we need more background info

-aimikins-

Thank you very much for your reply, yes it was primer dimer smile.gif

-fgalil-