real time PCR - getting high peak with c-ve (Dec/07/2005 )
hi! I'm getting very high peak of my c-ve in my real time PCR and when I run the products from capillaries in gel electrophoresis I don't get bands in c-ve please I need help!
-fgalil-
any help
-fgalil-
What is c-ve?
-Excentrifuge-
amplification peaks or melting curve peaks?
I bet it's primer dimer, but we need more background info
-aimikins-
Thank you very much for your reply, yes it was primer dimer
-fgalil-