3' - 5' activity of Taq DNA Polymerase - (Nov/25/2005 )

Anyone know anything at all about this residual activity of Taq DNA Polymerase. Any way of completely inhibiting it? Any heat stable polymerases that completely lack this activity?

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Please determine why you would like to remove the activity. If it affects the downstream experiment, you can use the PCR purification kit for removing it before moving onwards.

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It was to try to avoid any possible nibbling away at 3' end of an oligo. I don't know if this happens. It's just that there is a paper "Improvement of the 3'-5' Exonuclease activity of Taq DNA Polymerase ...." Mol Cells 7 419-424 Park et al (1997) and from the title I thought that there must be some sort of detectable activity to start with if you are going to do any improving.

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