From: Alberto
email: visintia@exchange.nih.gov
Category: RNA-Related

Message

hi there,

i appreciate if anybody with any successful experience in doing reverse northernscould provide me a reliable protocol for labeling cDNA (i use to use superscriptII) orshare with me his/her skills! thank you in advance, Alberto

Hi everybody,

I have done recently a subtractive library and I am trying to verify the clones with Reverse Northerns. Unfortunately with little success.I have a lot of troubles and I think the problem is the cDNA labeling.Does anybody has any suggestions? Is it to much if I ask for reliable protocol for labeling cDNA(radioactive or non-radiactive)?I would very much appreciate any help!

Kosmas