Warburg-christian equation - (Nov/06/2005 )
what is the formula of Warburg-Christian equation??
and what does it used for???
-strawberry-
no body have an idea?
-strawberry-
QUOTE (strawberry @ Nov 6 2005, 08:45 PM)
no body have an idea?
i don't have the formular at hand, but i guess google will help on that. the idea is, that you measure absorption at 260nm (nucleic acid) and 280nm (protein) of a sample. by comparison a formular (Warburg-Christian) was developed, that will give you the conncentration of nucleic acid and protein in that sample.
this is particular useful, if you want to judge if a nucleic acid sample is free of proteins.
hope that helps
-Kersten-
QUOTE (Kersten @ Nov 6 2005, 11:54 AM)
QUOTE (strawberry @ Nov 6 2005, 08:45 PM)
no body have an idea?
i don't have the formular at hand, but i guess google will help on that. the idea is, that you measure absorption at 260nm (nucleic acid) and 280nm (protein) of a sample. by comparison a formular (Warburg-Christian) was developed, that will give you the conncentration of nucleic acid and protein in that sample.
this is particular useful, if you want to judge if a nucleic acid sample is free of proteins.
hope that helps
The formula is Protein concentration (mg/ml)= (1.55*A2 80)-(0.76*A 260)
-Hanna-
hi
8th link on google results webpage for "Warburg-Christian equation". But you need a pdf reader...
-fred_33-