LacZ sequence - (Oct/18/2005 )
Hi,
need help!!!!
I'm lookin for the sequnce of Lacz and Neo, I need to know restriction enzyme in those seqeunce
need help!!!!
I'm lookin for the sequnce of Lacz and Neo, I need to know restriction enzyme in those seqeunce
i have the sequence of neo and I think you can get the Laz sequence from the commertial vectors.
i love people who said please..
anyway i get neo :
in this sequence, Neo starts at 66 and ends at 860. Use it in pDRAW (free software) to know any restriction enzyme.
1 CTAGGCTTTT GCAAAAAGCT TGATTCTTCT GACACAACAG TCTCGAACTT
51 AAGGCTAGAG CCACCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC
101 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
151 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG
201 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA
251 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC
351 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA
401 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
451 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT
501 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA
551 TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT
651 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT
701 GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
751 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT
801 ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA
851 GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC
901 CAACCTGCCA TCACGATGGC CGCAATAAAA TATCTTTATT TTCATTACAT
951 CTGTGTGTTG GTTTTTTGTG TGAATCGATA GCGATAAG
Restriction analysis, taking in consideration sites that are palindromic non degenerated and non interrupted, for 6bp or longer, with 5' or 3' overhangs or blunt ends :
Restriction analysis 18/10/2005
pDRAW32 revision 1.1.86
Rebase containing 257 enzymes.
18 enzymes match enzyme selection criteria.
********************************************************
AflII (C'TTAA_G) [BfrI,BspTI,Bst98I,MspCI,Vha464I]
Cuts 1 time.
Cuts at position 48.
Fragment sizes 48, 940.
BbeI (G_GCGC'C) [NarI,EgeI,EheI,KasI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 197.
Fragment sizes 197, 791.
BssHII (G'CGCG_C) [BsePI,PauI]
Cuts 1 time.
Cuts at position 591.
Fragment sizes 591, 397.
BstBI (TT'CG_AA) [AsuII,Bpu14I,Bsp119I,BspT104I,Csp45I,NspV,SfuI]
Cuts 1 time.
Cuts at position 876.
Fragment sizes 876, 112.
ClaI (AT'CG_AT) [BanIII,Bsa29I,BseCI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ZhoI]
[dam methylated]
Cuts 1 time.
Cuts at position 975.
Fragment sizes 975, 13.
EagI (C'GGCC_G) [BseX3I,BstZI,EclXI,Eco52I]
Cuts 1 time.
Cuts at position 100.
Fragment sizes 100, 888.
FspI (TGC'GCA) [Acc16I,AviII,NsbI]
Cuts 1 time.
Cuts at position 296.
Fragment sizes 296, 692.
HindIII (A'AGCT_T)
Cuts 1 time.
Cuts at position 16.
Fragment sizes 16, 972.
KasI (G'GCGC_C) [NarI,BbeI,EgeI,EheI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 193.
Fragment sizes 193, 795.
MscI (TGG'CCA) [BalI,MlsI,MluNI,Msp20I]
[dcm methylated]
Cuts 1 time.
Cuts at position 276.
Fragment sizes 276, 712.
NaeI (GCC'GGC) [MroNI,NgoMIV,PdiI]
Cuts 1 time.
Cuts at position 696.
Fragment sizes 696, 292.
NarI (GG'CG_CC) [BbeI,EgeI,EheI,KasI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 194.
Fragment sizes 194, 794.
NcoI (C'CATG_G) [Bsp19I]
Cuts 1 time.
Cuts at position 626.
Fragment sizes 626, 362.
NgoMIV (G'CCGG_C) [NaeI,MroNI,PdiI]
Cuts 1 time.
Cuts at position 694.
Fragment sizes 694, 294.
PstI (C_TGCA'G) [BspMAI]
Cuts 1 time.
Cuts at position 247.
Fragment sizes 247, 741.
PvuII (CAG'CTG)
Cuts 1 time.
Cuts at position 300.
Fragment sizes 300, 688.
SfoI (GGC'GCC) [NarI,BbeI,EgeI,EheI,KasI,Mly113I]
Cuts 1 time.
Cuts at position 195.
Fragment sizes 195, 793.
SphI (G_CATG'C) [BbuI,PaeI,SpaHI]
Cuts 1 time.
Cuts at position 599.
Fragment sizes 599, 389.
Restriction analysis, taking in consideration sites that are palindromic or not, degenerated or not and interrupted or not, for 4bp or longer, with 5' or 3' overhangs or blunt ends :
Restriction analysis 18/10/2005
pDRAW32 revision 1.1.86
Rebase containing 257 enzymes.
119 enzymes match enzyme selection criteria.
********************************************************
AcuI (CTGAAGnnnnnnnnnnnnnn_nn') [Eco57I]
Cuts 2 times.
Cuts at positions 340, 772.
Fragment sizes 340, 432, 216.
AflII (C'TTAA_G) [BfrI,BspTI,Bst98I,MspCI,Vha464I]
Cuts 1 time.
Cuts at position 48.
Fragment sizes 48, 940.
AluI (AG'CT)
Cuts 3 times.
Cuts at positions 18, 300, 758.
Fragment sizes 18, 282, 458, 230.
AlwI (GGATCnnnn'n_) [AclWI,BspPI]
[dam methylated]
Cuts 3 times.
Cuts at positions [373], [438], [619].
Fragment sizes 373, 65, 181, 369.
AvaII (G'GwC_C) [Bme18I,Eco47I,SinI,VpaK11BI]
[dcm methylated]
Cuts 1 time.
Cuts at position 710.
Fragment sizes 710, 278.
BanI (G'GyrC_C) [AccB1I,BshNI,BspT107I]
Cuts 2 times.
Cuts at positions 193, 228.
Fragment sizes 193, 35, 760.
BanII (G_rGCy'C) [Eco24I,EcoT38I,FriOI]
Cuts 1 time.
Cuts at position 559.
Fragment sizes 559, 429.
BbeI (G_GCGC'C) [NarI,EgeI,EheI,KasI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 197.
Fragment sizes 197, 791.
BccI (CCATCnnnn'n_)
Cuts 5 times.
Cuts at positions 72, 414, 500, 909, 916.
Fragment sizes 72, 342, 86, 409, 7, 72.
BceAI (ACGGCnnnnnnnnnnnn'nn_)
Cuts 2 times.
Cuts at positions 153, 620.
Fragment sizes 153, 467, 368.
BciVI (GTATCCnnnnn_n') [BfuI]
Cuts 1 time.
Cuts at position 413.
Fragment sizes 413, 575.
BfaI (C'TA_G) [MaeI,FspBI,XspI]
Cuts 2 times.
Cuts at positions 1, 55.
Fragment sizes 1, 54, 933.
Bme1580I (G_kGCm'C) [BseSI]
Cuts 2 times.
Cuts at positions 140, 233.
Fragment sizes 140, 93, 755.
BmrI (ACTGGGnnnn_n') [BfiI]
Cuts 1 time.
Cuts at position 143.
Fragment sizes 143, 845.
BplI (GAGnnnnnCTCnnnnnnnn_nnnnn')
Cuts 1 time.
Cuts at position 882.
Fragment sizes 882, 106.
BpuEI (CTTGAGnnnnnnnnnnnnnn_nn')
Cuts 1 time.
Cuts at position 570.
Fragment sizes 570, 418.
BsaAI (yAC'GTr) [BstBAI]
Cuts 1 time.
Cuts at position 498.
Fragment sizes 498, 490.
BsaHI (Gr'CG_yC) [AcyI,BstACI,Hin1I,Hsp92I]
Cuts 2 times.
Cuts at positions 194, 896.
Fragment sizes 194, 702, 92.
BsaJI (C'CnnG_G) [BseDI,BssECI]
Cuts 2 times.
Cuts at positions 357, 626.
Fragment sizes 357, 269, 362.
BsaWI (w'CCGG_w)
Cuts 1 time.
Cuts at position 225.
Fragment sizes 225, 763.
BseMII (CTCAGnnnnnnnn_nn') [BspCNI]
Cuts 1 time.
Cuts at position 848.
Fragment sizes 848, 140.
BseYI (C'CCAG_C)
Cuts 1 time.
Cuts at position 698.
Fragment sizes 698, 290.
BsiEI (CG_ry'CG) [Bsh1285I,BstMCI]
Cuts 1 time.
Cuts at position 103.
Fragment sizes 103, 885.
BsiHKAI (G_wGCw'C) [Alw21I,AspHI,Bbv12I]
Cuts 2 times.
Cuts at positions 307, 497.
Fragment sizes 307, 190, 491.
BslI (CCnn_nnn'nnGG) [BsiYI,Bsc4I,BseLI]
[dcm methylated]
Cuts 2 times.
Cuts at positions 175, 719.
Fragment sizes 175, 544, 269.
BslFI (GGGACnnnnnnnnnn'nnnn_) [BsmFI,FaqI]
Cuts 2 times.
Cuts at positions 345, 877.
Fragment sizes 345, 532, 111.
BslFI (GTCCCnnnnnnnnnnn_nnnn') [BsmFI,FaqI]
Cuts 2 times.
Cuts at positions 319, 851.
Fragment sizes 319, 532, 137.
BsmAI (GTCTCn'nnnn_) [Alw26I,BsoMAI]
Cuts 1 time.
Cuts at position 45.
Fragment sizes 45, 943.
BsmFI (GGGACnnnnnnnnnn'nnnn_) [BslFI,FaqI]
Cuts 2 times.
Cuts at positions 345, 877.
Fragment sizes 345, 532, 111.
Bsp1286I (G_dGCh'C) [SduI,BmyI,MhlI]
Cuts 5 times.
Cuts at positions 140, 233, 307, 497, 559.
Fragment sizes 140, 93, 74, 190, 62, 429.
BspCNI (CTCAGnnnnnnn_nn') [BseMII]
Cuts 1 time.
Cuts at position 849.
Fragment sizes 849, 139.
BspMI (ACCTGCnnnn'nnnn_) [Acc36I,BfuAI,BveI]
Cuts 3 times.
Cuts at positions 81, 462, 912.
Fragment sizes 81, 381, 450, 76.
BsrI (ACTG_Gn') [Bse1I,BseNI,BsrSI]
Cuts 2 times.
Cuts at positions 138, 339.
Fragment sizes 138, 201, 649.
BsrBI (CCG'CTC) [AccBSI,MbiI]
Cuts 2 times.
Cuts at positions 807, 861.
Fragment sizes 807, 54, 127.
BsrDI (GCAATG_nn') [Bse3DI,BseMI]
Cuts 1 time.
Cuts at position 427.
Fragment sizes 427, 561.
BsrFI (r'CCGG_y) [Cfr10I,Bse118I,BssAI]
Cuts 2 times.
Cuts at positions 513, 694.
Fragment sizes 513, 181, 294.
BssHII (G'CGCG_C) [BsePI,PauI]
Cuts 1 time.
Cuts at position 591.
Fragment sizes 591, 397.
BssSI (C'ACGA_G) [BauI,Bst2BI]
Cuts 1 time.
Cuts at position 786.
Fragment sizes 786, 202.
BstBI (TT'CG_AA) [AsuII,Bpu14I,Bsp119I,BspT104I,Csp45I,NspV,SfuI]
Cuts 1 time.
Cuts at position 876.
Fragment sizes 876, 112.
BstF5I (GGATG_nn') [FokI,BseGI]
Cuts 2 times.
Cuts at positions 511, 536.
Fragment sizes 511, 25, 452.
BstKTI (G_AT'C) [MboI,BfuCI,Bsp143I,BstMBI,DpnII,Kzo9I,NdeII,Sau3AI]
Cuts 5 times.
Cuts at positions 368, 446, 527, 536, 614.
Fragment sizes 368, 78, 81, 9, 78, 374.
BstNI (CC'w_GG) [EcoRII,AjnI,BptI,BseBI,BstOI,Bst2UI,MvaI,Psp6I,PspGI]
Cuts 1 time.
Cuts at position 581.
Fragment sizes 581, 407.
BstUI (CG'CG) [AccII,Bsh1236I,BstFNI,MvnI]
Cuts 3 times.
Cuts at positions 260, 561, 593.
Fragment sizes 260, 301, 32, 395.
BstXI (CCAn_nnnn'nTGG)
Cuts 1 time.
Cuts at position 915.
Fragment sizes 915, 73.
BstYI (r'GATC_y) [XhoII,BstX2I,MflI,PsuI]
Cuts 2 times.
Cuts at positions 365, 611.
Fragment sizes 365, 246, 377.
BtgI (C'CryG_G) [BstDSI]
Cuts 1 time.
Cuts at position 626.
Fragment sizes 626, 362.
BtgZI (GCGATGnnnnnnnnnn'nnnn_)
Cuts 3 times.
Cuts at positions 465, 646, 809.
Fragment sizes 465, 181, 163, 179.
ClaI (AT'CG_AT) [BanIII,Bsa29I,BseCI,Bsp106I,BspDI,BspXI,Bsu15I,BsuTUI,ZhoI]
[dam methylated]
Cuts 1 time.
Cuts at position 975.
Fragment sizes 975, 13.
Csp6I (G'TA_C) [RsaI,AfaI]
Cuts 1 time.
Cuts at position 499.
Fragment sizes 499, 489.
CspCI (CAAnnnnnGTGGnnnnnnnnnn_nn')
Cuts 1 time.
Cuts at position 50.
Fragment sizes 50, 938.
CspCI (CCACnnnnnTTGnnnnnnnnnnn_nn')
Cuts 1 time.
Cuts at position 85.
Fragment sizes 85, 903.
CviAII (C'AT_G) [NlaIII,FatI,Hin1II,Hsp92II]
Cuts 5 times.
Cuts at positions 65, 410, 596, 627, 653.
Fragment sizes 65, 345, 186, 31, 26, 335.
DdeI (C'TnA_G) [BstDEI]
Cuts 1 time.
Cuts at position 857.
Fragment sizes 857, 131.
DpnI (GA'TC)
Cuts 5 times.
Cuts at positions 367, 445, 526, 535, 613.
Fragment sizes 367, 78, 81, 9, 78, 375.
DrdI (GACnn_nn'nnGTC) [AasI,DseDI]
Cuts 2 times.
Cuts at positions 37, 221.
Fragment sizes 37, 184, 767.
EaeI (y'GGCC_r) [CfrI]
[dcm methylated]
Cuts 5 times.
Cuts at positions 100, 274, 665, 692, 917.
Fragment sizes 100, 174, 391, 27, 225, 71.
EagI (C'GGCC_G) [BseX3I,BstZI,EclXI,Eco52I]
Cuts 1 time.
Cuts at position 100.
Fragment sizes 100, 888.
EarI (CTCTTCn'nnn_) [Ksp632I,Bst6I,Eam1104I]
Cuts 2 times.
Cuts at positions 538, 748.
Fragment sizes 538, 210, 240.
EciI (GGCGGAnnnnnnnnn_nn')
Cuts 1 time.
Cuts at position 723.
Fragment sizes 723, 265.
Eco57MI (CTGrAGnnnnnnnnnnnnnn_nn')
Cuts 2 times.
Cuts at positions 340, 772.
Fragment sizes 340, 432, 216.
FalI (AAGnnnnnCTTnnnnnnnn_nnnnn')
Cuts 1 time.
Cuts at position 534.
Fragment sizes 534, 454.
FatI ('CATG_) [NlaIII,CviAII,Hin1II,Hsp92II]
Cuts 5 times.
Cuts at positions 64, 409, 595, 626, 652.
Fragment sizes 64, 345, 186, 31, 26, 336.
FauI (CCCGCnnnn'nn_) [SmuI]
Cuts 2 times.
Cuts at positions 317, 854.
Fragment sizes 317, 537, 134.
FokI (GGATGnnnnnnnnn'nnnn_) [BseGI,BstF5I]
Cuts 2 times.
Cuts at positions 518, 543.
Fragment sizes 518, 25, 445.
FspI (TGC'GCA) [Acc16I,AviII,NsbI]
Cuts 1 time.
Cuts at position 296.
Fragment sizes 296, 692.
HaeII (r_GCGC'y) [Bsp143II,BstH2I]
Cuts 1 time.
Cuts at position 197.
Fragment sizes 197, 791.
HaeIII (GG'CC) [BshFI,BspANI,BsuRI,PalI,PhoI]
Cuts 5 times.
Cuts at positions 102, 276, 667, 694, 919.
Fragment sizes 102, 174, 391, 27, 225, 69.
HgaI (GACGCnnnnn'nnnnn_)
Cuts 1 time.
Cuts at position 904.
Fragment sizes 904, 84.
Hin4I (GAynnnnnvTCnnnnnnnn_nnnnn')
Cuts 3 times.
Cuts at positions 357, 387, 389.
Fragment sizes 357, 30, 2, 599.
Hin4I (GAbnnnnnrTCnnnnnnnn_nnnnn')
Cuts 3 times.
Cuts at positions 357, 389, 419.
Fragment sizes 357, 32, 30, 569.
HindIII (A'AGCT_T)
Cuts 1 time.
Cuts at position 16.
Fragment sizes 16, 972.
HinfI (G'AnT_C)
Cuts 5 times.
Cuts at positions 22, 679, 813, 865, 972.
Fragment sizes 22, 657, 134, 52, 107, 16.
HphI (GGTGAnnnnnnn_n') [AsuHPI]
[dam methylated]
Cuts 1 time.
Cuts at position 372.
Fragment sizes 372, 616.
Hpy99I (_CGwCG')
Cuts 5 times.
Cuts at positions 284, 312, 606, 621, 898.
Fragment sizes 284, 28, 294, 15, 277, 90.
Hpy188I (TC_n'GA)
Cuts 4 times.
Cuts at positions 30, 161, 505, 858.
Fragment sizes 30, 131, 344, 353, 130.
HpyCH4III (AC_n'GT) [Bst4CI,TaaI]
Cuts 4 times.
Cuts at positions 39, 574, 690, 798.
Fragment sizes 39, 535, 116, 108, 190.
HpyCH4IV (A'CG_T) [MaeII,TaiI,TscI]
Cuts 2 times.
Cuts at positions 310, 497.
Fragment sizes 310, 187, 491.
HpyCH4V (TG'CA) [CviRI]
Cuts 5 times.
Cuts at positions 11, 86, 245, 420, 434.
Fragment sizes 11, 75, 159, 175, 14, 554.
KasI (G'GCGC_C) [NarI,BbeI,EgeI,EheI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 193.
Fragment sizes 193, 795.
MaeIII ('GTnAC_)
Cuts 2 times.
Cuts at positions 314, 620.
Fragment sizes 314, 306, 368.
MboI ('GATC_) [BfuCI,Bsp143I,BstKTI,BstMBI,DpnII,Kzo9I,NdeII,Sau3AI]
[dam methylated]
Cuts 5 times.
Cuts at positions [365], [443], [524], [533], [611].
Fragment sizes 365, 78, 81, 9, 78, 377.
MboII (GAAGAnnnnnnn_n')
[dam methylated]
Cuts 4 times.
Cuts at positions 17, 555, 765, 845.
Fragment sizes 17, 538, 210, 80, 143.
MlyI (GAGTCnnnnn') [PleI,PpsI,SchI]
Cuts 1 time.
Cuts at position 859.
Fragment sizes 859, 129.
MnlI (CCTCnnnnnn_n')
Cuts 4 times.
Cuts at positions 109, 245, 602, 795.
Fragment sizes 109, 136, 357, 193, 193.
MscI (TGG'CCA) [BalI,MlsI,MluNI,Msp20I]
[dcm methylated]
Cuts 1 time.
Cuts at position 276.
Fragment sizes 276, 712.
MseI (T'TA_A) [Tru1I,Tru9I]
Cuts 1 time.
Cuts at position 49.
Fragment sizes 49, 939.
MslI (CAynn'nnrTG) [SmiMI]
Cuts 3 times.
Cuts at positions 631, 913, 952.
Fragment sizes 631, 282, 39, 36.
MspA1I (CmG'CkG)
Cuts 1 time.
Cuts at position 300.
Fragment sizes 300, 688.
NaeI (GCC'GGC) [MroNI,NgoMIV,PdiI]
Cuts 1 time.
Cuts at position 696.
Fragment sizes 696, 292.
NarI (GG'CG_CC) [BbeI,EgeI,EheI,KasI,Mly113I,SfoI]
Cuts 1 time.
Cuts at position 194.
Fragment sizes 194, 794.
NciI (CC's_GG) [AsuC2I,BcnI]
Cuts 2 times.
Cuts at positions 198, 358.
Fragment sizes 198, 160, 630.
NcoI (C'CATG_G) [Bsp19I]
Cuts 1 time.
Cuts at position 626.
Fragment sizes 626, 362.
NgoMIV (G'CCGG_C) [NaeI,MroNI,PdiI]
Cuts 1 time.
Cuts at position 694.
Fragment sizes 694, 294.
NlaIII (_CATG') [CviAII,FatI,Hin1II,Hsp92II]
Cuts 5 times.
Cuts at positions 68, 413, 599, 630, 656.
Fragment sizes 68, 345, 186, 31, 26, 332.
NlaIV (GGn'nCC) [BspLI,PspN4I]
Cuts 2 times.
Cuts at positions 195, 230.
Fragment sizes 195, 35, 758.
NspI (r_CATG'y) [BstNSI,XceI]
Cuts 1 time.
Cuts at position 599.
Fragment sizes 599, 389.
PleI (GAGTCnnnn'n_) [MlyI,PpsI,SchI]
Cuts 1 time.
Cuts at position 859.
Fragment sizes 859, 129.
PspGI ('CCwGG_) [EcoRII,AjnI,BptI,BseBI,BstNI,BstOI,Bst2UI,MvaI,Psp6I]
Cuts 1 time.
Cuts at position 579.
Fragment sizes 579, 409.
PstI (C_TGCA'G) [BspMAI]
Cuts 1 time.
Cuts at position 247.
Fragment sizes 247, 741.
PvuII (CAG'CTG)
Cuts 1 time.
Cuts at position 300.
Fragment sizes 300, 688.
RsaI (GT'AC) [AfaI,Csp6I]
Cuts 1 time.
Cuts at position 500.
Fragment sizes 500, 488.
RsrII (CG'GwC_CG) [CpoI,CspI,Rsr2I]
Cuts 1 time.
Cuts at position 710.
Fragment sizes 710, 278.
SapI (GCTCTTCn'nnn_)
Cuts 2 times.
Cuts at positions 538, 748.
Fragment sizes 538, 210, 240.
Sau96I (G'GnC_C) [AspS9I,Cfr13I,PspPI]
[dcm methylated]
Cuts 1 time.
Cuts at position 710.
Fragment sizes 710, 278.
ScrFI (CC'n_GG) [Bme1390I,BssKI,BstSCI,MspR9I,StyD4I]
[dcm methylated]
Cuts 3 times.
Cuts at positions 198, 358, [581].
Fragment sizes 198, 160, 223, 407.
SfcI (C'TryA_G) [BfmI,BpcI,BstSFI]
Cuts 1 time.
Cuts at position 243.
Fragment sizes 243, 745.
SfoI (GGC'GCC) [NarI,BbeI,EgeI,EheI,KasI,Mly113I]
Cuts 1 time.
Cuts at position 195.
Fragment sizes 195, 793.
SmlI (C'TyrA_G)
Cuts 2 times.
Cuts at positions 48, 585.
Fragment sizes 48, 537, 403.
SphI (G_CATG'C) [BbuI,PaeI,SpaHI]
Cuts 1 time.
Cuts at position 599.
Fragment sizes 599, 389.
StyI (C'CwwG_G) [BssT1I,Eco130I,EcoT14I,ErhI]
Cuts 1 time.
Cuts at position 626.
Fragment sizes 626, 362.
StyD4I ('CCnGG_) [ScrFI,Bme1390I,BssKI,BstSCI,MspR9I]
Cuts 3 times.
Cuts at positions 196, 356, 579.
Fragment sizes 196, 160, 223, 409.
TaiI (_ACGT') [MaeII,HpyCH4IV,TscI]
Cuts 2 times.
Cuts at positions 313, 500.
Fragment sizes 313, 187, 488.
TaqII (GACCGAnnnnnnnnn_nn')
Cuts 2 times.
Cuts at positions 231, 899.
Fragment sizes 231, 668, 89.
TaqII (CACCCAnnnnnnnnn_nn')
Cuts 2 times.
Cuts at positions 98, 690.
Fragment sizes 98, 592, 298.
TfiI (G'AwT_C) [PfeI]
Cuts 4 times.
Cuts at positions 22, 679, 813, 972.
Fragment sizes 22, 657, 134, 159, 16.
Tsp45I ('GTsAC_) [NmuCI]
Cuts 2 times.
Cuts at positions 314, 620.
Fragment sizes 314, 306, 368.
TspDTI (ATGAAnnnnnnnnn_nn')
Cuts 3 times.
Cuts at positions 253, 671, 931.
Fragment sizes 253, 418, 260, 57.
TspRI (_nnCAsTGnn')
Cuts 2 times.
Cuts at positions 323, 323.
Fragment sizes 323, 0, 665.
Tth111I (GACn'n_nGTC) [AspI,PflFI,PsyI,TelI]
Cuts 1 time.
Cuts at position 312.
Fragment sizes 312, 676.
please please pretty please
thanks a lot 
sorry for that.
I let my personal problems interfer with the forum.
Sorry