Transfecting primary cortical neurons - (Oct/12/2005 )
I just found a reply to a query about transfecting cells with JET PEI which prompted me to register so I could find out some more information.
I am also looking for an efficient way of transfecting primary cortical neurons and will definitely try Gencarrier-2 as was recommended. I was wondering if anyone could give me a few other details about primary neuron transfections - do you transfect at plating or in older cultures? Have you ever tried to transfect long term static cultures (i.e. 1-2 weeks)? What sort of medium are you using for your cortical cultures?
I don't need a huge transfection efficiency but I am aiming for a good representative sample and I need to maintain high survival as I want to look at synapse development.
Any information would be greatly appreciated,
thanks and best wishes,
Jenny Gunnersen
Howard Florey Institute
j.gunnersen@hfi.unimelb.edu.au
I am also looking for an efficient way of transfecting primary cortical neurons and will definitely try Gencarrier-2 as was recommended. I was wondering if anyone could give me a few other details about primary neuron transfections - do you transfect at plating or in older cultures? Have you ever tried to transfect long term static cultures (i.e. 1-2 weeks)? What sort of medium are you using for your cortical cultures?
I don't need a huge transfection efficiency but I am aiming for a good representative sample and I need to maintain high survival as I want to look at synapse development.
Any information would be greatly appreciated,
thanks and best wishes,
Jenny Gunnersen
Howard Florey Institute
j.gunnersen@hfi.unimelb.edu.au
Hi Jenny
I'm also looking for an efficient way for transfecting Neurons, i tryed Lipfectamine, Fugene and Calcium precipitation untill now, but the eficiency is too low for me, i want to try JetPei, do have any additional information about it?