Condition for amplifying >6k PCR - Long PCR amplification (Oct/11/2005 )
I managed to get it twice... then i didn't get it anymore.
-axisy-
Hi,
You may try to reduce the denaturation time to 10sec.
-Henry-
hi
did you try with 2%DMSO in your mix?
2% is in majority not inhibiting and helps to prevent non specificity.
-fred_33-