What's the difference MS/MS and MALDI-TOF? - (Oct/07/2005 )
Can anyone tell me (briefly)? Thanks 
i'm not a physician, and don't do currently with MS, but i got few month ago info from a colleague...
Maldi-TOF : matrix assisted laser desorbtion ionization-time of flight....
Maldi is a technique of breaking molecules as electrospray is. Time of flight signifies that you have quite a tunnel, and the detector is at the end of the tunnel. Changes in Magnetic field drives one specific molecule to have a long "fly" into the tunnel and reach te detector.
MS/MS means that you do a first break of your moleacule A. You'll get peptides analysed. Second run by the machine, it breaks your first molecule, eliminate all peptides except one, and do an other break on it, and analyses it. The MSMS allows more precision, if i'm not wrong.
Fred
Do you mean....
In MALDI-TOF, you will have a bunch of peptide mass, but not in order.
In MS/MS, you will have peptide mass line up according to their order.
Is that right?
Maldi-TOF : matrix assisted laser desorbtion ionization-time of flight....
Maldi is a technique of breaking molecules as electrospray is. Time of flight signifies that you have quite a tunnel, and the detector is at the end of the tunnel. Changes in Magnetic field drives one specific molecule to have a long "fly" into the tunnel and reach te detector.
MS/MS means that you do a first break of your moleacule A. You'll get peptides analysed. Second run by the machine, it breaks your first molecule, eliminate all peptides except one, and do an other break on it, and analyses it. The MSMS allows more precision, if i'm not wrong.
Fred