about 3T3 and serum - (Oct/03/2005 )

Hi,

I am now culturing 3T3 cells. This cell supposed to be very strong. But my cell is in bad state It has a lot of bubbles in side and out side of the cells. I found in some protocol, this cell should keep in 10% bovine calf serum, not fetal bovine serum. But I only have fetal serum now. Is that the cause of my poor cells?

Thanks

-agarosey-

I'm not sure what your problem is but 3T3's will grow Better in fetal compared to calf serum.

-ajames-

you can try to decomplement your serum by heating it at 56° for 30'
fred

-fred_33-

QUOTE (agarosey @ Oct 3 2005, 11:01 PM)

Hi,

I am now culturing 3T3 cells. This cell supposed to be very strong. But my cell is in bad state

It has a lot of bubbles in side and out side of the cells. I found in some protocol, this cell should keep in 10% bovine calf serum, not fetal bovine serum. But I only have fetal serum now. Is that the cause of my poor cells?

Thanks

Recently we were facing the same problem. So we have been testing 6 different batches of 3T3 cells (they all have a different morphology) and it turned our that they all grow fine on Newborn Calf Serum 9% (heat inactivated) but very bad on Fetal Calf serum (9%)

-Theo22-

I have seen this also and it has varied depending upon where I got my cells from. It is amazing that 3T3 cells seem to vary so much depending upon the lab you got them from, but this seems to be the case.

-tap14-

All the different 3T3s not only differ in their serum requirements. Also in their state of transformation, stress response, growthrate etc.

-Theo22-

I'll ditto that statement. That is why you need to take 3T3 transformation studies with a grain of salt or at least know where they got "their" 3T3 cells from

-tap14-