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stem cells and EB (Embryoid bodies) - (Aug/25/2009 )

Anybody have problems with stem cells culture and EB contamination? Lately I have been experiencing lots of contamination, my entire batches of HESC and EB have all died!! My hard work have all gone down the drain!!! :~( What could it be due to!!!???

Another things, is the b-fgf very crucial for the stem cell differentiation respressing? I seem to have like add abit less than expected


PLS HELP!!!!

-ursae-

ursae on Tue Aug 25 07:38:13 2009 said:


Anybody have problems with stem cells culture and EB contamination? Lately I have been experiencing lots of contamination, my entire batches of HESC and EB have all died!! My hard work have all gone down the drain!!! :~( What could it be due to!!!???

Another things, is the b-fgf very crucial for the stem cell differentiation respressing? I seem to have like add abit less than expected


PLS HELP!!!!


do you mean contamination with bacteria/fungi?

-vitalgene-