CHO or VERO? - Cell culture (Aug/13/2009 )
Trying to express fusion protein. I have searched ATCC extensively. Just need a simple answer- which is preferred, easiest to work with?
Thanks!
-AReslow-
both are easy to work with, and should be fine for this sort of thing. Having said that, if you want to purify the protein, express it in bacteria.
-bob1-
Thanks Bob,
I have tired to express in bacteria and have had little luck. Granted I have not tried too hard. It is a small, highly folded protein. We have made modifications to avoid signal cleavage and secretion. I have performed Westerns on cells and sup (just in case it was secreted). Hoping all mammalian cells will work better for expression.
-AReslow-