Hammerhead ribozyme cleavage - (Aug/13/2009 )

Hi,

Does anybody know the protocol of Hammerhead ribozyme clevage?and how to design the ribozyme?

Sorry I don't have much. Here's a paper on inhibiting a ribozyme; the references might be useful for design.

Yen L, Svendsen J, Lee JS, Gray JT, Magnier M, Baba T, D'Amato RJ, Mulligan RC. Exogenous control of mammalian gene expression through modulation of RNA self-cleavage. Nature. 2004 Sep 23;431(7007):471-6.

Good hunting,

- Jon

Let's make it clear. Ur trying to get a hyammerhead ribozyme. Is that right?
What I know is you can use SELEX to screen it. I don't know how to design it.