In vitro screening for antitumoral activity - wich tests? (Aug/12/2009 )

Hi
I was wondering: wich tests would you suggest if you have a set of compounds that you want to screen for cytotoxic activity on some cancer cell lines
Thanks

Canotto,
One resource may be the PubChem database. A largenumber of viablity/cytotoxicity assays have been deposited here.

Regards,
Kevin

Promega Corporation

canotto on Aug 12 2009, 09:33 PM said:

You could start with the trypan blue exclusion assay to see if there's any change in cell growth rates, its cheap and easy but not good for high throughput.
http://www.fishersci.com/wps/downloads/seg..._Protocol_3.pdf

Apoptosis assays include annexin V binding, caspase activity, BrdU incorporation, DNA laddering, and many more.....
http://www.apoptosisworld.com/ApoptosisAssays.html

You could also look for senescence using beta-galactosidase activity.
http://www.scienceboard.net/resources/prot...protocol_id=120

P

Penguin on Aug 13 2009, 11:39 AM said:

thanks,
so the tests that I know and that can be useful in this setting are:
Cell Viability assays ( like Trypan Blue, or 7AAD or PI)
Cell Proliferation assays ( like the CFSE assay)
Cell Cycle Analysis tests ( like Rnase/PI in fixed cells)
Apoptosis assays ( like the Annexin V assay)
Senescence assays

do you know something equivalent, or at least comparable, to the MTT assay, that I can perform in flow cytometry?