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cloudy gel - (Aug/02/2009 )

I have prepared a 15% gel but after polymerization it bocomes cloudy what should I do?

-parinaz-

Determine what it is that is making it cloudy, I am guessing precipitation of something. Prepare fresh solutions and try again.

-bob1-

did you prepare all the solutions including acrylamide?

did you add the right amount of bisacrylamide to the acrylamide solution?

a relatively high percentage of bisacrylamide will turn the gel cloudy when polymerized.

-mdfenko-