Making standard dilution for qPCR - (Jul/28/2009 )

Hi, all

I found one article about qPCR. In that article, the authors made 10-fold serial dilution of their plasmid control with nuclease-free water containing carrier tRNA (100 ng/microliter)

Is cRNA critical for making serial dilution?

If anyone has information about cRNA for making DNA serial dilution, please share your information.



Here is the link to that article.

http://www.pubmedcentral.nih.gov/articlere...bmedid=15933012

thank god, Im doing qPCR and RT-PCR and any literature is great! sorry i was no help