lactose tetrazolium - (Jul/21/2009 )

does anyone here have experience in the use of lactose tetrazolium agar? can you please share your recipe? Thanks

There's a good discussion of its application at : NATURE REVIEWS | GENETICS VOLUME 4 | JUNE 2003 | 419 ART AND DESIGN OF GENETIC SCREENS: ESCHERICHIA COLI

Ref for prep.: Beckwith, J. Restoration of operon activity by suppressors. Biochim. Biophys. Acta 76, 162–164 (1963).

Thanks. i have that paper, however there was no mention of the recipe...just the principles. The Beckwith paper is not available in PDF

Contact Bruce Levin - think he's at Emory now.