culturing COS-7 cells - (Jul/17/2009 )

Hi all,

I am having serious problems in culturing COS-7 cells.. they tend to maintain the same state , as in, they neither grow or die off after getting adhered..morphology is fine..n the intriguin part is that they they are growin in parallel lines? HELP PLEASE

proteome on Jul 17 2009, 09:16 AM said:

I find that my COS-7 cells grow quite rapidly and do so in a normal pattern with the expected morphology. I have never seen them fail to grow or grow in "lines." Two questions, then:
1) Are you using the proper medium? I use low glucose DMEM supplemented with 10% fetal bovine serum
2) Are you sure these are COS-7 cells?

Hi,

Does your medium contain glutamine? Or do you have to add it? Did you? This was the reason why some of my cells did not grow, thought medium contains l-glutamin /stabilized glutamin but it did not...

Stardust

stardust on Jul 18 2009, 07:47 AM said:

Yes, the medium contains L-glutamine and 110 mg/L sodium pyruvate.

Dr Teeth on Jul 20 2009, 03:08 PM said:

yours might - but its a little too easy to grab the wrong dmem bottle if your new to the game - proteome - you should check

love the idea of growth in parralel lines - like synchronised swimming for cells - you could sell tickets

dom

Hmmm, parallel line usually means that your cell cultures are being exposed to vibrations - check your incubator for a small vibration and get it sorted out. See attachment.