how to make standard curve of tyrosine. - (Jul/17/2009 )

Anyone plz help to find out the comlete method of making tyrosine standard curve for alkaline protease assay...

Think following might help.

One unit of the alkaline protease activity (U) was defined as the amount of enzyme liberating 1 μg of tyrosine per min per ml under the assay conditions
Production of alkaline protease from an alkaliphilic actinomycete
Bioresource Technology
Volume 97, Issue 14, September 2006, Pages 1650-1654