contamination - contamination (Jul/16/2009 )
Hi all,
i have a problem that there is signal in negative control in real time PCR?why is it caused?i use new reagents but contamination is found.what should i do?please help me.
best regards,
thinsu
-thinsu-
On possible cause is the formation of primer dimers. For this, one of the best (and most time efficient) approaches is to redesign your assay and try again. I typically design at least three assays, test them all together, and chose the best one.
Good luck
-ivanbio-
ivanbio on Jul 16 2009, 07:23 AM said:
On possible cause is the formation of primer dimers. For this, one of the best (and most time efficient) approaches is to redesign your assay and try again. I typically design at least three assays, test them all together, and chose the best one.
Good luck
Good luck
thanks a lot. i 'll try it.
best regards,
thinsu
-thinsu-