GC rich DNA sequencing - (Jul/14/2009 )

Does anyone have any successful experience regarding sequencing GC rich (~70%) DNA sequence. I tried with 3% DMSO. Sequencing always stop once meet GC rich region. Thanks a lot.

try 1M betaine

mdfenko on Jul 14 2009, 03:33 PM said:

I also see this online too. Do you change anything of sequencing cycle? Thanks.

nope. add the betaine and run as usual.

i often tell investigators to increase the number of cycles to maximum when dealing with weak reactions.