cell loss upon centrifugatino - (Jul/14/2009 )
abohollolo on Aug 6 2009, 12:01 PM said:
little mouse on Aug 2 2009, 11:53 PM said:
I had human samples. I never weight them.
I don't know how to help you. Do you filter the cells? (you should not)
I didn't. I put the pieces of tissue (after digestion and wash) in a culture flask and wait for the cells to grow outside of the tissue.
I don't know how to help you. Do you filter the cells? (you should not)
I didn't. I put the pieces of tissue (after digestion and wash) in a culture flask and wait for the cells to grow outside of the tissue.
Why shouldnt i filter the cell?
i filter for chondrocytes and fibroblasts and is working well
anyway. i used smaller tubes (15 ml) and is giving better results.
thanks for the answers
I filtered once, to do better than best, but I lost a lot of cells. I got better results by putting the tissues and after two passages I had pure FLS
-little mouse-