How to prepapre 1,25-Dihydroxyvitamin D3 (calcitriol) - (Jul/07/2009 )
Hi,
I need to prepapre teh 10-8M of 1,25-Dihydroxyvitamin D3 (molecular weight: 416.64) in ethanol for cell culture. I've ordered the chemical from Sigma but they only deliver 10ug and 0.1mg volumes. I have ordered 10ug. Has anyone used this before. What form does it come in?
I'm having trouble figuring out the small quantity. Can someone help me with the calculations please? If its made in ethanol, then I will have to filter sterilise it, cannot be autoclaved.
The manual says: For use in cell culture, this product was diluted into medium from 10 μM stock solutions prepared in ethanol. So shall I make 10uk stock solution first?
Thanks.
SF_HK on Jul 7 2009, 01:49 AM said:
I need to prepapre teh 10-8M of 1,25-Dihydroxyvitamin D3 (molecular weight: 416.64) in ethanol for cell culture. I've ordered the chemical from Sigma but they only deliver 10ug and 0.1mg volumes. I have ordered 10ug. Has anyone used this before. What form does it come in?
I'm having trouble figuring out the small quantity. Can someone help me with the calculations please? If its made in ethanol, then I will have to filter sterilise it, cannot be autoclaved.
The manual says: For use in cell culture, this product was diluted into medium from 10 μM stock solutions prepared in ethanol. So shall I make 10uk stock solution first?
Thanks.
You need to dissolve the 10ug in 2.4 ml ethanol, if I understand it right.
For recipe calculators, see these tools. Make sure to use right units (use grams and moles, instead of ug, uM).
cellcounter on Jul 8 2009, 01:15 AM said:
SF_HK on Jul 7 2009, 01:49 AM said:
I need to prepapre teh 10-8M of 1,25-Dihydroxyvitamin D3 (molecular weight: 416.64) in ethanol for cell culture. I've ordered the chemical from Sigma but they only deliver 10ug and 0.1mg volumes. I have ordered 10ug. Has anyone used this before. What form does it come in?
I'm having trouble figuring out the small quantity. Can someone help me with the calculations please? If its made in ethanol, then I will have to filter sterilise it, cannot be autoclaved.
The manual says: For use in cell culture, this product was diluted into medium from 10 μM stock solutions prepared in ethanol. So shall I make 10uk stock solution first?
Thanks.
You need to dissolve the 10ug in 2.4 ml ethanol, if I understand it right.
For recipe calculators, see these tools. Make sure to use right units (use grams and moles, instead of ug, uM).
Thank you. What concentration would this give me?