Transcription - Quality of DNA (Jul/02/2009 )

HI ALL,

IF anyone can suggest me the protocol for obtaining highly purified DNA sample for transcription and transcription protocol as well
I am using Nucleobond Kit protocol for plasmid DNA preparation, and for transcription, i used linearized fragment post-elution from the agarose gel. If anyone has suggestions. Please write about it

Thanks

Preeti

-preeti_RNA-

preeti_RNA on Jul 2 2009, 07:40 PM said:

if you want to express your gene in E.coli, then you can insert your gene into expression vector which you can select using relative antibiotic. after you can get you want

-zhenhuowuyan-