FPLC vs. HPLC - (Jul/01/2009 )
I am planning to purchase either HPLC or FPLC for protein purification.
Does anyone have a suggestion regarding which one is better?
OR, is it possible to use HPLC as FPLC just by switching a column?
Thanks.
I saw once semi-prep-size gel filtration column connected to Shimadzu HPLC system and it worked quite well. I think salts commonly used in protein purification methods (gel filtration, ion exchange, hydrophopic interaction) may reduce lifetime of HPLC pumps unless you have pump/piston washing set.
In my opinion - if you don't plan to use reversed phase columns, especially in analytical scale, buy FPLC. If you plan or hope to use your system for semi-prep or analytical RP-HPLC (besides its usual FPLC protein purification) - buy HPLC.
we use a biocompatible hplc to run fplc columns.
this gives us more flexibility in methods development and we require only one instrument, rather than two.
mdfenko on Jul 1 2009, 12:10 PM said:
this gives us more flexibility in methods development and we require only one instrument, rather than two.
I'm fairly certain that's what the NMR lab at my old institute does as well.
is basically the same...
nothing wrong for you to use HPLC to do fractionation
but you have to becareful the the limit of your pump....
quantanary pump can not support semi-prep separation....
in my lab we are using two single pump working as normal HPLC and also FPLC...
If it is only for protein purification, I would recommend going for FPLC. I think the range of columns that can be attached is much more but check once.
Best,
TC
parkc23 on Jul 1 2009, 01:51 PM said:
Does anyone have a suggestion regarding which one is better?
OR, is it possible to use HPLC as FPLC just by switching a column?
Thanks.