Plant RNA extraction problem - A cry for help. (Jun/25/2009 )
Hello all,
I've been trying to extract RNA form an Arabidopsis tissue using TRI Reagent (Sigma). It has been four times now and nothing. First three times I did it with 100 mg of tissue, seeing that it did not work, I went up to 1 g and followed the same procedure adjusting the volumes accordingly. Again, nothing, not even degraded RNA is seen in the gel. Funny thing is that when I used 100 mg of different tissues, I succesfully extracted the RNA. I don't know what is goning on with his one.
Any ideas?
Thanks in advance.
-Pancho-
look at this topic from the archives:
rna extraction from plant tissue
-mdfenko-
mdfenko on Jun 26 2009, 06:53 AM said:
nice topic! i'm so thankful with the protocol...
------------------
Science News and Reasearch
-analynsarte-