Adherent cells versus suspension cells - how can i convert adherent cell culture to suspension culture (Jun/16/2009 )

My qyestion is I have an adherent cell line and I want to convert the same cell line to be used for suspension culture

Why do you want to convert? to culture more cells in suspension? then you can culture them on beads.

little mouse on Jun 16 2009, 01:48 PM said:

dr talal on Jun 16 2009, 02:08 PM said:

I want to convert so I can scale up. Scaling up is limited when it comes to roller bottles so i want to use reactors. Will beads help and how

dr talal on Jun 16 2009, 12:38 PM said:

I never did it myself, but I know that one can scale up the production of adherent cells by growing them on beads, beads being in suspension in the medium. your cells are still adherent cells, but you can increase the number of cells per liter of medium.

little mouse on Jun 16 2009, 03:58 PM said:

Can you suggest beads supplier and any other technical details on how to grow them and so forth. Many thanks

sorry I can't. I have no experience in that field. Only theoritical knowledge.
But I'm quite sure that someone will reply if you re-title your post.