Transgene copy number - (Jun/08/2009 )
Can any body share their protocol or provide me a link to read how to estimate transgene copy numebr?
I have never done it but understand that it can be done by southern blot or real time PCR. Real time PCR seems to be less cumbersome.
I have never done real time PCR as well but use PCR very often in my project.
Thanks for your help!
-res_org-
res_org on Jun 8 2009, 08:08 AM said:
Can any body share their protocol or provide me a link to read how to estimate transgene copy numebr?
I have never done it but understand that it can be done by southern blot or real time PCR. Real time PCR seems to be less cumbersome.
I have never done real time PCR as well but use PCR very often in my project.
Thanks for your help!
I have never done it but understand that it can be done by southern blot or real time PCR. Real time PCR seems to be less cumbersome.
I have never done real time PCR as well but use PCR very often in my project.
Thanks for your help!
had also to quantify vetor/particle in my transfection experiments! think this is right, but should be confirmed!! see attachment. with that you can form a standard curve with a vector stock (e.g. 50ng/reaction) -> you know how many copies you have in 50ng...
-moljul-