diluting to final primer concentration - please help correct (Jun/07/2009 )

hello,
I have a tube of forward and reverse primer at 100uM concentration, in volume of 50ul.

I need to get both primers to to 50nmol in 100ul final.

How do I do this?

-biogirl1230-

biogirl1230 on Jun 7 2009, 12:45 PM said:

hello,
I have a tube of forward and reverse primer at 100uM concentration, in volume of 50ul.

I need to get both primers to to 50nmol in 100ul final.

How do I do this?

You would need 50 nl. It would be best to dilute your primer stock to say 1 uM with a 1:100 dilution and then use 5 ul of this in your 100 ul final reaction.

-Dr Teeth-