spectrophotometer-problem with adjusting autozero - Aconitase assay - problem with adjusting autozero (Jun/02/2009 )

Dear all,

I am doing aconitase assay and the wavelength to be used is 240 nm and the spectrophotometer which I use is Shimadzu UV-2410 PC spectrophotometer. The reaction mixture contains Tris-HCl and isocitrate and I am facing some problem with adjusting autozero. The reading is not at all getting stable. I tried to adjust autozero with only Tris-HCl, but the result is same. Can anyone help me to solve this problem.

Thanks a bunch in advance,

Have a nice day,
Bindu

-sbindu-

sbindu on Jun 3 2009, 05:33 AM said:

Do you allow at least 30 minutes for the spectro to warm up before using? If so then I would guess that the deuterium lamp probably needs changing. It is often forgotten that these have a limited lifespan compared to the tungsten one used in the visible range.

Hope this helps

-klinmed-

we also had same problem once, we got it calibrated. I don't know about technical details :lol: . And yes its always better to switch on the instrument at least 10-15 mins prior to use.

-amj2-

Thanks a lot for the suggestion...

amj2 on Jun 5 2009, 11:55 PM said:

we also had same problem once, we got it calibrated. I don't know about technical details

. And yes its always better to switch on the instrument at least 10-15 mins prior to use.

-sbindu-

Thank you so much for the help....

klinmed on Jun 2 2009, 05:16 PM said:

sbindu on Jun 3 2009, 05:33 AM said:

-sbindu-