Drosophila tissue homogenization - need help (Jun/02/2009 )
I'm trying to extract proteins and RNAs from adult whole Drosophilas. At the moment I'm using an IKA ultra turrax blender, which is not a tissue homogenizer per se. I would really appreciate if someone could give advice on the best methods on homogenizing Drosophila tissues. Thanks a lot in advance.
-abbas-
abbas on Jun 2 2009, 05:57 AM said:
I'm trying to extract proteins and RNAs from adult whole Drosophilas. At the moment I'm using an IKA ultra turrax blender, which is not a tissue homogenizer per se. I would really appreciate if someone could give advice on the best methods on homogenizing Drosophila tissues. Thanks a lot in advance.
Abbas,
I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.
Cheers,
AquaPlasmid
-AquaPlasmid-
AquaPlasmid on Jun 3 2009, 04:48 PM said:
Abbas,
I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.
Cheers,
AquaPlasmid
I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.
Cheers,
AquaPlasmid
Thanks for the reply. I rinsed the blender with 70% ethanol between each homogenization. Is bleach better than using ethanol?
-abbas-
abbas on Jun 3 2009, 12:59 PM said:
AquaPlasmid on Jun 3 2009, 04:48 PM said:
Abbas,
I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.
Cheers,
AquaPlasmid
I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.
Cheers,
AquaPlasmid
Thanks for the reply. I rinsed the blender with 70% ethanol between each homogenization. Is bleach better than using ethanol?
Yes, bleach! In my hands only bleach rinse can prevent cross-contamination.
-AquaPlasmid-