volume of DNA required (PCr product ) in agarose gel - (Jun/01/2009 )
jiajia1987 on Jun 3 2009, 01:47 AM said:
if you want to know the concentration of your PCR products, you can always make use of a nanodrop, or a spectrophotometer.
Only if you have cleaned them up, free dNTPs interfere with this reading.
-bob1-
bob1 on Jun 4 2009, 08:06 AM said:
jiajia1987 on Jun 3 2009, 01:47 AM said:
if you want to know the concentration of your PCR products, you can always make use of a nanodrop, or a spectrophotometer.
Only if you have cleaned them up, free dNTPs interfere with this reading.
yep. either by DNA cleanup or gel extraction.
-jiajia1987-