ELISA Optimization - Plate Choice (May/27/2009 )
I was using a 96 well flexible roundbottom plate and getting very high positive readings of around 3 (abs). I switched to flat bottom, high-binding strip plates and now my absorbance for my positives has dropped to around 2.
Any help on the loss of OD?
-blake.r-
It is very normal, different materials plates have different bingding capacity, so have different OD. If you want have stabilized results,maybe you should use the same material's plate in your experience always.
-tinaking-
Elisa flat bottom nunc or corning
-sgt4boston-