cloning miRNA library - (May/27/2009 )

I'm planning to isolate and clone miRNAs from cells lines under two different conditions - to compare those conditions in regard to which miRNA are specific to a condition.
However, I have no prior experience with miRNA methods.
After doing some readings it is my understanding that there are two main strategies for cloning miRNA: 1. the adaptor ligation
2. using polyadenilation and polyT-adaptor primer for RT reaction.
Which one is better in regards to efficiency?
Which one is easier?
I would greatly appreciate if someone could give me a protocol that works.

Thanks to all
root

If miRNA profiling is what you want to study differential miRNA expression between two cell lines or conditions. Then, miRNA microarray or realtime RT-PCR might save you time (and troubles as well).

Functional Screens on May 27 2009, 10:16 PM said:

Or high throughput sequencing if you want to find novel miRNAs.

miRNA man on May 28 2009, 07:43 AM said:

Thank you for your former answers- so I started looking into this and I'm currently checking costs. So my next question is- for high throughput sequncing- how many biological repeats do I need? and for the microarray?

Genome Res. (2008) 18:610 Application of massively parallel sequencing to microRNA profiling and discovery in human embryonic stem cells.

For array, it's up to you to do duplicate, triplicate, or more........