MMP 9 isolation using sepharose beads - tagging gelatin to sepharose beads (May/25/2009 )
hello every one,
i need to purify MMP9 from conditioned media so i need gelatin tagged to sepharose beads. So any one have protocol how gelatin is tagged to sepharose beads.
thanks in advance,
alie.
-smilealie-
Wouldn't it be better to immunoprecipitate with an antibody and protein A or G sepharose beads?
-bob1-