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MMP 9 isolation using sepharose beads - tagging gelatin to sepharose beads (May/25/2009 )

hello every one,

i need to purify MMP9 from conditioned media so i need gelatin tagged to sepharose beads. So any one have protocol how gelatin is tagged to sepharose beads.

thanks in advance,
alie.

-smilealie-

Wouldn't it be better to immunoprecipitate with an antibody and protein A or G sepharose beads?

-bob1-