DMEM vs. DMEM F12 - Difference !? (May/06/2009 )

thank you for your confirmation about the freezing ;

what if i used dmem/f12 with phenol red then due to some problem, i used the same media but without phenol red. is that any risk ?

Potentially - phenol red is a weak estrogen analogue...

bob1 on Wed Nov 28 08:48:26 2012 said:

A weak estrogen analogue is a substance that activates the estrogen receptor and will cause internalization etc. THe weakness comes from needing quite a bit of the phenol red there for this to happen. Most phenol-red containing mediums have enough to activate the receptor, so if you are studying the effects of estrogen or something that may be affected by estrogen (breast cells, ovaries etc.), use phenol-red free medium.